Composite

Part:BBa_K2737016

Designed by: Xinyu Teng   Group: iGEM18_ECUST   (2018-10-15)


Plac-mcherry-Pfur1-lacI

An iron invert system. There is a fur box between -35 and -10 region. When ferric iron concentration rises in cytoplasm, Fur can bind with fux box and repress expression of lacI, then mcherry can be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None